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Job Title: Research Technician II-III, Immunotherapy
Company Name: Fred Hutchinson Cancer Research Center
Location: Seattle, WA US
Position Type: Full Time
Post Date: 10/27/2017
Expire Date: 11/26/2017
Job Categories: Computers, Software, Healthcare, Other, Information Technology, Collegiate Faculty, Staff, Administration, Research & Development, Medical, Nonprofit and Volunteer Services
Job Description
Research Technician II-III, Immunotherapy


The Program in Immunology in the Clinical Research Division is looking for a highly motivated individual to support the development of cancer immunotherapies. The successful candidate will utilize a variety of cutting-edge cellular and molecular techniques to analyze T cell immunity to cancer. (S)he will be closely involved in the preclinical development of new T cell receptor (TCR)-based therapies and their translation into clinical trials, and should be capable of maintaining detailed laboratory records, analyzing data, and reporting experimental results.


Cures Start Here. Fred Hutchinson Cancer Research Center (Fred Hutch), home of three Nobel laureates, is an independent, nonprofit research institution dedicated to the development and advancement of biomedical research to eliminate cancer and other potentially fatal diseases. Recognized internationally for its pioneering work in bone-marrow transplantation, Fred Hutch’s five scientific divisions collaborate to form a unique environment for conducting basic and applied science. The Fred Hutch, in collaboration with its clinical and research partners, the University of Washington and Seattle Children’s, is the only National Cancer Institute-designated comprehensive cancer center in the Pacific Northwest. Careers Start Here.


Assist in the design, implementation and interpretation of experiments involving human T lymphocytes engineered to target tumor cells. These studies are specifically aimed at identifying new antigenic targets to broaden the scope of engineered T cell therapy of cancer. Some mouse T cell experiments may also be performed to assess the safety and efficacy of candidate TCRs.


Perform tissue culture and molecular biology techniques: Generation of antigen-specific T cells, isolation of genetic material and amplification and sequencing of T cell receptor genes, transduction with retroviral and lentiviral vectors, FACS sorting and expanding cells in vitro.


Isolate and culture primary hematopoietic cells from human peripheral blood of healthy donors and leukemia patients.


Analyze T cell phenotype and function using techniques including flow cytometry, proliferation assays, PCR, RT-PCR, sequencing, and T cell cytolytic assays.


Assist with the management and organization of a fast-paced lab. Other duties as assigned by supervisor will be performed as necessary including conducting literature reviews to assist in determining most suitable methods to be used in research.


Contribute to the preparation and presentation of written or oral progress reports concerning studies and assist in publication of results.


Minimum Qualifications:


Tech II level: A bachelor’s degree in a biological field and two years or more of post-bachelor’s laboratory research experience with molecular biology techniques.

Tech III level: A bachelor’s degree in a biological field and five years or more of post-bachelor’s laboratory research experience with molecular biology techniques


To succeed in this position candidate must enjoy working independently but be able to interact well in a team environment.


Excellent organizational and interpersonal skills, the ability to multi-task, and attention to detail are critical.




Immunology background with particular knowledge of T lymphocyte biology and MHC-restriction would be ideal


Experience in aseptic cell culture techniques, multi-color flow cytometry and in establishing and maintaining T-cell lines/clones is preferred.


Molecular biology expertise: bacterial cloning, lentiviral/retroviral transduction min 2 year career experience.


Master’s degree a plus.


Computer skills: Experience with Microsoft Excel, Prism (Graphpad), FlowJo, DNA/gene analysis software and statistical programs for data analysis.

Our Commitment to Diversity

We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We are proud to be an Equal Opportunity and VEVRAA Employer. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability, marital or veteran status, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at escmail@fredhutch.org or by calling 206-667-4700.

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Company Name: Fred Hutchinson Cancer Research Center
Company Description:

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